《植物生理学报》 2017, 53(7): 1287-1296

定量蛋白质组学方法揭示受体蛋白激酶EMS1调控拟南芥花药发育的下游信号网络

陆佳楠, 叶娟英, 许俊旭, 马红, 常芳^*

复旦大学生命科学学院植物科学研究所, 上海200438

通信作者：常；E-mail: fangchang@fudan.edu.cn

摘　要：

被子植物的花药是产生雄配子的器官, 其正常发育对植物的有性生殖和正常繁衍至关重要。在花药发育的过程中, 受体激酶EMS1 (EXCESS MICROSPOROCYTES 1)及其胞外配基TPD1 (TAPETUM DETERMINANT 1)通过调控花药绒毡层的发育而对雄性育性发挥重要作用。然而目前对于TPD1–EMS1的下游信号转导网络却知之甚少。随着质谱和同位素标记技术的发展, 定量蛋白质组学在植物研究中的应用越来越广泛, 在深入研究生物学过程的分子表型以及揭示蛋白调控网络方面发挥着越来越重要的作用。本文利用基于iTRAQ技术的定量蛋白质组学, 以野生型和ems1及tpd1突变体1~9期花苞为材料, 通过蛋白质组数据及转录组数据的对比分析, 深入挖掘了TPD1–EMS1调控花药发育过程中下游信号网络, 找到了一大批受TPD1、EMS1共同或特异调控的已知和未知功能的蛋白, 为深入研究花药发育的调控网络提供了新的认识和重要的参考。

关键词：花药发育; 绒毡层; TPD1; EMS1; 定量蛋白质组学; iTRAQ

收稿：2017-05-27   修定：2017-06-05

资助：国家自然科学基金(31130006)。
致谢 复旦大学生命科学学院遗传工程国家重点实验室质谱平台对课题提供设备和技术支持。

Quantitative proteomics analysis reveals the signaling networks downstream of EMS1 in the regulation of anther development in Arabidopsis

LU Jia-Nan, YE Juan-Ying, XU Jun-Xu, MA Hong, CHANG Fang^*

Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China

Corresponding author: CHANG Fang; E-mail: fangchang@fudan.edu.cn

Abstract:

The anthers of angiosperms are organs that produce male gametes, and their normal development is essential for sexual reproduction and plant multiply. The receptor-like kinase EMS1 (EXCESS MICROSPOROCYTES 1) and its extracellular ligand TPD1 (TAPETUM DETERMINANT 1) play important roles in the male fertility by regulating the development of anther tapetum during anther development. However, little is known about the signal transduction network downstream of TPD1–EMS1. With the development of mass spectrometry and isotope labeling technologies, quantitative proteomics has been widely used in plant research and has played an increasingly important role in further studying the molecular phenotype of biological processes and revealing protein regulatory networks. In this paper, iTRAQ-based proteomics analysis was used to compare the proteomic data and transcriptome data of stage 1–9 floral buds from the wild type, ems1 and tpd1 mutants. The results uncovered components of signaling networks, including a large number of proteins with known or unknown functions that they are regulated by TPD1 and EMS1, and provide a new understanding and important reference for further study of the regulation network of anther development.

Key words: anther development; tapetum; TPD1; EMS1; quantitative proteomics; iTRAQ